Structural Biology of Peptides¶
Understanding peptide structure at atomic resolution is fundamental to rational drug design. This section covers the principal experimental and computational methods used to determine peptide three-dimensional conformations.
Peptide Secondary Structure Determination
Alpha-helices, beta-sheets, turns, and random coils — principles, prediction methods, and experimental validation of peptide secondary structure.
X-ray Crystallography of Peptides
Crystallization strategies, data collection, phase determination, and refinement for peptide crystal structures at atomic resolution.
NMR Spectroscopy for Peptide Structure
Solution-state NMR methods for peptide structure determination, including 2D experiments, chemical shift assignment, and distance restraint calculation.
Circular Dichroism in Peptide Analysis
Principles and applications of far-UV and near-UV CD spectroscopy for peptide secondary structure estimation, folding studies, and thermal stability analysis.
Peptide–Protein Interaction Structures
Methods for determining peptide-receptor and peptide-protein complex structures, including co-crystallization, docking, and integrative approaches.